A federal judge blocked Theranos Inc. founder Elizabeth Holmes's proposal to ask potential jurors more than 100 questions before her criminal-fraud trial, saying she could get a fair hearing without making inquiries that prosecutors called deeply intrusive.

U.S. District Judge Edward Davila countered Tuesday with a slimmed-down questionnaire to send to jurors ahead of the late-August trial, which will determine whether Ms. Holmes defrauded investors, patients and doctors about her now-defunct blood-testing company. 

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Prosecutors allege that Ms. Holmes and the company's former chief operating officer, Ramesh "Sunny" Balwani, overstated the abilities of Theranos's technology, which purported to test for dozens of health conditions with a few drops of blood extracted from a finger prick. Both have pleaded not guilty and could face up to 20 years in prison if convicted. 

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